研究领域

1.各种重要膜蛋白及酶类等的结构功能。

2.生物活性物质（抗肿瘤，抗炎，抗菌等）的合成机制、调控、改造。

3.微生物的分子遗传学。

4.具有重要应用前景的环境微生物的分离，鉴定等方向。

工作经历

2007-至今，威廉希尔williamhill官方网站，教授

承担课题

细菌六型分泌系统基板复合体TssF-G-K的结构解析及TssK与系统其它核心蛋白相互作用的研究 (2018.1-2021.12)，国家自然科学基金，主持

光驱动质子泵变形菌视紫红质（Proteorhodopsins）的晶体结构及质子转运机理的解析 (2012.1-2015.12)，国家自然科学基金，主持

霍乱弧菌草酰乙酸脱羧酶晶体结构与其脱羧和钠离子跨膜转运功能体现 (2010.1-2012.12) ，国家自然科学基金，主持

Proteorhodopsin的分子结构及其质子转运功能的研究（2008.1-2010.12），国家自然科学基金青年基金，主持

膜蛋白位点2蛋白酶的晶体结构解析及功能的研究（2012.8-2014.7），中央高校基本科研业务费，主持

YidC对浑球红细菌光合系统膜生物发生及能量转换的研究（2010.1-2012.12），教育部重点项目，主持

发表论文：

1: Xu D, Gao Y, Sun B, Ran T, Zeng L, He J, He J, Wang W. Pyrethroid
Carboxylesterase PytH from <i>Sphingobium faniae</i> JZ-2: Structure and
Catalytic Mechanism. Appl Environ Microbiol. 2020 Jun 2;86(12):e02971-19. doi:
10.1128/AEM.02971-19. PMID: 32303545; PMCID: PMC7267200.

2: Ran T, Li W, Sun B, Xu M, Qiu S, Xu DQ, He J, Wang W. Crystal structure of
mature myroilysin and implication for its activation mechanism. Int J Biol
Macromol. 2020 Aug 1;156:1556-1564. doi: 10.1016/j.ijbiomac.2019.11.205. Epub
2019 Nov 28. PMID: 31785296.

3: Chen R, Yu Y, Feng Z, Gan R, Xie X, Zhang Z, Xie Q, Wang W, Ran T, Zhang W,
Xiong Q, Shao G. Featured Species-Specific Loops Are Found in the Crystal
Structure of <i>Mhp</i> Eno, a Cell Surface Adhesin From <i>Mycoplasma
hyopneumoniae</i>. Front Cell Infect Microbiol. 2019 Jun 13;9:209. doi:
10.3389/fcimb.2019.00209. PMID: 31263685; PMCID: PMC6585157.

4: Wang X, Sun B, Xu M, Qiu S, Xu D, Ran T, He J, Wang W. Crystal structure of
the periplasmic domain of TssL, a key membrane component of Type VI secretion
system. Int J Biol Macromol. 2018 Dec;120(Pt B):1474-1479. doi:
10.1016/j.ijbiomac.2018.09.166. Epub 2018 Sep 26. PMID: 30266644.

5: Li P, Xu D, Ma T, Wang D, Li W, He J, Ran T, Wang W. Crystal structures of
the kinase domain of PpkA, a key regulatory component of T6SS, reveal a general
inhibitory mechanism. Biochem J. 2018 Jul 17;475(13):2209-2224. doi:
10.1042/BCJ20180077. PMID: 29858276.

6: Wang X, Ran T, Zhang X, Xin J, Zhang Z, Wu T, Wang W, Cai G. 3.9 Å structure
of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP. Science. 2017 Dec
1;358(6367):1206-1209. doi: 10.1126/science.aan8414. PMID: 29191911.

7: Zhang X, Wu D, Guo T, Ran T, Wang W, Xu D. Differential roles for ArcA and
ArcB homologues in swarming motility in Serratia marcescens FS14. Antonie Van
Leeuwenhoek. 2018 Apr;111(4):609-617. doi: 10.1007/s10482-017-0981-9. Epub 2017
Nov 14. PMID: 29139003.

8: Chen X, Ji J, Zhao L, Qiu J, Dai C, Wang W, He J, Jiang J, Hong Q, Yan X.
Molecular Mechanism and Genetic Determinants of Buprofezin Degradation. Appl
Environ Microbiol. 2017 Aug 31;83(18):e00868-17. doi: 10.1128/AEM.00868-17.
PMID: 28710269; PMCID: PMC5583499.

9: Zhang Y, Qiu S, Jia S, Xu D, Ran T, Wang W. Crystal structure of the sensor
domain of BaeS from Serratia marcescens FS14. Proteins. 2017
Sep;85(9):1784-1790. doi: 10.1002/prot.25326. Epub 2017 Jun 7. PMID: 28544098.

10: Zhang F, Wei Q, Tong H, Xu D, Wang W, Ran T. Crystal structure of MBP-PigG
fusion protein and the essential function of PigG in the prodigiosin
biosynthetic pathway in Serratia marcescens FS14. Int J Biol Macromol. 2017
Jun;99:394-400. doi: 10.1016/j.ijbiomac.2017.02.088. Epub 2017 Mar 1. PMID:
28258005.

11: Xu D, Zhou J, Lou X, He J, Ran T, Wang W. Myroilysin Is a New Bacterial
Member of the M12A Family of Metzincin Metallopeptidases and Is Activated by a
Cysteine Switch Mechanism. J Biol Chem. 2017 Mar 31;292(13):5195-5206. doi:
10.1074/jbc.M116.758110. Epub 2017 Feb 9. PMID: 28188295; PMCID: PMC5392667.

12: Wu D, Li P, Zhou J, Gao M, Lou X, Ran T, Wu S, Wang W, Xu D. Identification
of a toxic serralysin family protease with unique thermostable property from S.
marcescens FS14. Int J Biol Macromol. 2016 Dec;93(Pt A):98-106. doi:
10.1016/j.ijbiomac.2016.08.041. Epub 2016 Aug 16. PMID: 27543346.

13: Wei Q, Ran T, Ma C, He J, Xu D, Wang W. Crystal Structure and Function of
PqqF Protein in the Pyrroloquinoline Quinone Biosynthetic Pathway. J Biol Chem.
2016 Jul 22;291(30):15575-87. doi: 10.1074/jbc.M115.711226. Epub 2016 May 26.
PMID: 27231346; PMCID: PMC4957043.

14: Wang X, Chu H, Lv M, Zhang Z, Qiu S, Liu H, Shen X, Wang W, Cai G. Structure
of the intact ATM/Tel1 kinase. Nat Commun. 2016 May 27;7:11655. doi:
10.1038/ncomms11655. PMID: 27229179; PMCID: PMC4894967.

15: Wu D, Ran T, Wang W, Xu D. Structure of a thermostable serralysin from
Serratia sp. FS14 at 1.1 Å resolution. Acta Crystallogr F Struct Biol Commun.
2016 Jan;72(Pt 1):10-5. doi: 10.1107/S2053230X15023092. Epub 2016 Jan 1. PMID:
26750478; PMCID: PMC4708044.

16: Zhou H, Li P, Wu D, Ran T, Wang W, Xu D. EheA from Exiguobacterium sp. yc3
is a novel thermostable DNase belonging to HNH endonuclease superfamily. FEMS
Microbiol Lett. 2015 Dec;362(24):fnv204. doi: 10.1093/femsle/fnv204. Epub 2015
Oct 22. PMID: 26500089.

17: Xu D, Gao Y, Wang P, Ran T, Wang W. Presence of an amino acid residue at
position 619 required for the function of YidC in Rhodobacter sphaeroides.
Biochem Biophys Res Commun. 2015 Oct 16;466(2):267-71. doi:
10.1016/j.bbrc.2015.09.032. Epub 2015 Sep 8. PMID: 26362178.

18: Li P, Kwok AH, Jiang J, Ran T, Xu D, Wang W, Leung FC. Comparative genome
analyses of Serratia marcescens FS14 reveals its high antagonistic potential.
PLoS One. 2015 Apr 9;10(4):e0123061. doi: 10.1371/journal.pone.0123061. PMID:
25856195; PMCID: PMC4391916.

19: Ran T, Gao M, Wei Q, He J, Tang L, Wang W, Xu D. Expression, crystallization
and preliminary crystallographic data analysis of VioD, a hydroxylase in the
violacein-biosynthesis pathway. Acta Crystallogr F Struct Biol Commun. 2015
Feb;71(Pt 2):149-52. doi: 10.1107/S2053230X14027617. Epub 2015 Jan 28. PMID:
25664787; PMCID: PMC4321467.

20: Han N, Ran T, Lou X, Gao Y, He J, Tang L, Xu D, Wang W. Expression,
crystallization and preliminary crystallographic data analysis of PigI, a
putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia.
Acta Crystallogr F Struct Biol Commun. 2014 May;70(Pt 5):624-7. doi:
10.1107/S2053230X14005780. Epub 2014 Apr 17. PMID: 24817724; PMCID: PMC4014333.

21: Lou X, Ran T, Han N, Gao Y, He J, Tang L, Xu D, Wang W. Crystal structure of
the catalytic domain of PigE: a transaminase involved in the biosynthesis of
2-methyl-3-n-amyl-pyrrole (MAP) from Serratia sp. FS14. Biochem Biophys Res
Commun. 2014 Apr 25;447(1):178-83. doi: 10.1016/j.bbrc.2014.03.125. Epub 2014
Apr 2. PMID: 24704447.

22: Ran T, Ozorowski G, Gao Y, Sineshchekov OA, Wang W, Spudich JL, Luecke H.
Cross-protomer interaction with the photoactive site in oligomeric
proteorhodopsin complexes. Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt
10):1965-80. doi: 10.1107/S0907444913017575. Epub 2013 Sep 20. PMID: 24100316.

23: Wu J, Li J, Mao X, Wang W, Cheng Z, Zhou Y, Zhou X, Tao X. Viroplasm protein
P9-1 of Rice black-streaked dwarf virus preferentially binds to single-stranded
RNA in its octamer form, and the central interior structure formed by this
octamer constitutes the major RNA binding site. J Virol. 2013
Dec;87(23):12885-99. doi: 10.1128/JVI.02264-13. Epub 2013 Sep 25. PMID:
24067964; PMCID: PMC3838130.

24: Zhu W, Lan Y, Lou X, Han N, Ran T, Xu L, Xu D, Wang WW. Isolation of
proteorhodopsin-bearing bacterium JL-3 from fresh water and characterization of
the proteorhodopsin. FEMS Microbiol Lett. 2013 Jul;344(1):10-7. doi:
10.1111/1574-6968.12144. Epub 2013 May 1. PMID: 23551202.

25: Li Y, Zhu H, Zeng X, Fan J, Qian X, Wang S, Wang Z, Sun Y, Wang X, Wang W,
Ju D. Suppression of autophagy enhanced growth inhibition and apoptosis of
interferon-β in human glioma cells. Mol Neurobiol. 2013 Jun;47(3):1000-10. doi:
10.1007/s12035-013-8403-0. Epub 2013 Jan 18. PMID: 23329343.

26: Ran T, Gao Y, Marsh M, Zhu W, Wang M, Mao X, Xu L, Xu D, Wang W. Crystal
structures of Cg1458 reveal a catalytic lid domain and a common catalytic
mechanism for the FAH family. Biochem J. 2013 Jan 1;449(1):51-60. doi:
10.1042/BJ20120913. PMID: 23046410.

27: Liu S, Ran T, Shen X, Xu L, Wang W, Xu D. Expression, crystallization and
preliminary crystallographic data analysis of PigF, an O-methyltransferase from
the prodigiosin-synthetic pathway in Serratia. Acta Crystallogr Sect F Struct
Biol Cryst Commun. 2012 Aug 1;68(Pt 8):898-901. doi: 10.1107/S1744309112024001.
Epub 2012 Jul 31. PMID: 22869117; PMCID: PMC3412768.

28: Wang N, Wang M, Gao Y, Ran T, Lan Y, Wang J, Xu L, Wang W. Crystallization
and preliminary X-ray crystallographic analysis of a blue-light-absorbing
proteorhodopsin. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Mar
1;68(Pt 3):281-3. doi: 10.1107/S1744309111043612. Epub 2012 Feb 22. PMID:
22442222; PMCID: PMC3310530.

29: Huang L, Cao RB, Wang N, Liu K, Wei JC, Isahg H, Song LJ, Zuo WY, Zhou B,
Wang WW, Mao X, Chen PY. The design and recombinant protein expression of a
consensus porcine interferon: CoPoIFN-α. Cytokine. 2012 Jan;57(1):37-45. doi:
10.1016/j.cyto.2011.10.011. Epub 2011 Nov 15. PMID: 22088600.

30: Ran T, Wang Y, Xu D, Wang W. Expression, purification, crystallization and
preliminary crystallographic analysis of Cg1458: a novel oxaloacetate
decarboxylase from Corynebacterium glutamicum. Acta Crystallogr Sect F Struct
Biol Cryst Commun. 2011 Aug 1;67(Pt 8):968-70. doi: 10.1107/S1744309111023220.
Epub 2011 Jul 27. PMID: 21821907; PMCID: PMC3151140.

31: Studer R, Dahinden P, Wang WW, Auchli Y, Li XD, Dimroth P. Crystal structure
of the carboxyltransferase domain of the oxaloacetate decarboxylase Na+ pump
from Vibrio cholerae. J Mol Biol. 2007 Mar 23;367(2):547-57. doi:
10.1016/j.jmb.2006.12.035. Epub 2006 Dec 19. PMID: 17270211.

32: Mao X, Wang W, Jiang W, Zhao GP. His23beta and Glu455beta of the Pseudomonas
sp. 130 glutaryl-7-amino cephalosporanic acid acylase are crucially important
for efficient autoproteolysis and enzymatic catalysis. Protein J. 2004
Apr;23(3):197-204. doi: 10.1023/b:jopc.0000026415.96041.27. PMID: 15200051.

33: Wang W, Gao J, Chiao J, Zhao G, Jiang W. A novel two-component system amrB-
amkB involved in the regulation of central carbohydrate metabolism in rifamycin
SV-producing Amycolatopsis mediterranei U32. Curr Microbiol. 2004
Jan;48(1):14-9. doi: 10.1007/s00284-003-3997-5. PMID: 15018097.

34: Wang WW, Sineshchekov OA, Spudich EN, Spudich JL. Spectroscopic and
photochemical characterization of a deep ocean proteorhodopsin. J Biol Chem.
2003 Sep 5;278(36):33985-91. doi: 10.1074/jbc.M305716200. Epub 2003 Jun 23.
PMID: 12821661.

35: Man D, Wang W, Sabehi G, Aravind L, Post AF, Massana R, Spudich EN, Spudich
JL, Béjà O. Diversification and spectral tuning in marine proteorhodopsins. EMBO
J. 2003 Apr 15;22(8):1725-31. doi: 10.1093/emboj/cdg183. PMID: 12682005; PMCID:
PMC154475.

36: Wang W, Zhang W, Chen H, Chiao J, Zhao G, Jiang W. Molecular and biochemical
characterization of a novel two-component signal transduction system, amrA-
amkA, involved in rifamycin SV production in Amycolatopsis mediterranei U32.
Arch Microbiol. 2002 Nov;178(5):376-86. doi: 10.1007/s00203-002-0468-5. Epub
2002 Aug 22. PMID: 12375106.