研究领域

微生物来源的酶类的结构与功能，细菌次级代谢产物的合成及调控机制。

主持或参加的各类科研项目

1. 国家自然科学基金青年基金，31400055，沙雷氏菌灵菌红素的前体MBC合成途径中相关蛋白结构解析及反应机制的研究，2015/01-2017/12，23万元，主持， 已结题。

2. 江苏省自然科学基金青年基金BK20140690，灵菌红素前体MBC合成途径相关酶的结构解析及机制的研究，2014/07-2017/06，20万元，主持，已结题。

3. 国家自然科学基金面上基金，31671800，鱼腥藻脂肪氧合酶催化机制及其分子改造研究，2017/01-2020/12，62万元，参加，在研。

4. 国家自然科学基金面上基金（小额资助），31470095，鱼腥藻脂肪氧合酶热稳定性提高及其分子机理研究，2015/01-2016/12，30万元，参加，已结题。

5. 国家自然科学基金青年基金，21302098，真菌漆酶催化Domino反应制备多羟基苯并呋喃类物质机理研究，2014/01-2016/12，25万元，参加，已结题。

教学经验

基础微生物学，基础微生物学实验，微生物生理学实验，微生物学大实验，生命科学导论

发表论文：

1: Xu D, Gao Y, Sun B, Ran T, Zeng L, He J, He J, Wang W. Pyrethroid
Carboxylesterase PytH from <i>Sphingobium faniae</i> JZ-2: Structure and
Catalytic Mechanism. Appl Environ Microbiol. 2020 Jun 2;86(12):e02971-19. doi:
10.1128/AEM.02971-19. PMID: 32303545; PMCID: PMC7267200.

2: Ran T, Li W, Sun B, Xu M, Qiu S, Xu DQ, He J, Wang W. Crystal structure of
mature myroilysin and implication for its activation mechanism. Int J Biol
Macromol. 2020 Aug 1;156:1556-1564. doi: 10.1016/j.ijbiomac.2019.11.205. Epub
2019 Nov 28. PMID: 31785296.

3: Chen R, Yu Y, Feng Z, Gan R, Xie X, Zhang Z, Xie Q, Wang W, Ran T, Zhang W,
Xiong Q, Shao G. Featured Species-Specific Loops Are Found in the Crystal
Structure of <i>Mhp</i> Eno, a Cell Surface Adhesin From <i>Mycoplasma
hyopneumoniae</i>. Front Cell Infect Microbiol. 2019 Jun 13;9:209. doi:
10.3389/fcimb.2019.00209. PMID: 31263685; PMCID: PMC6585157.

4: Wang X, Sun B, Xu M, Qiu S, Xu D, Ran T, He J, Wang W. Crystal structure of
the periplasmic domain of TssL, a key membrane component of Type VI secretion
system. Int J Biol Macromol. 2018 Dec;120(Pt B):1474-1479. doi:
10.1016/j.ijbiomac.2018.09.166. Epub 2018 Sep 26. PMID: 30266644.

5: Li P, Xu D, Ma T, Wang D, Li W, He J, Ran T, Wang W. Crystal structures of
the kinase domain of PpkA, a key regulatory component of T6SS, reveal a general
inhibitory mechanism. Biochem J. 2018 Jul 17;475(13):2209-2224. doi:
10.1042/BCJ20180077. PMID: 29858276.

6: Wang X, Ran T, Zhang X, Xin J, Zhang Z, Wu T, Wang W, Cai G. 3.9 Å structure
of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP. Science. 2017 Dec
1;358(6367):1206-1209. doi: 10.1126/science.aan8414. PMID: 29191911.

7: Zhang X, Wu D, Guo T, Ran T, Wang W, Xu D. Differential roles for ArcA and
ArcB homologues in swarming motility in Serratia marcescens FS14. Antonie Van
Leeuwenhoek. 2018 Apr;111(4):609-617. doi: 10.1007/s10482-017-0981-9. Epub 2017
Nov 14. PMID: 29139003.

8: Zhang Y, Qiu S, Jia S, Xu D, Ran T, Wang W. Crystal structure of the sensor
domain of BaeS from Serratia marcescens FS14. Proteins. 2017
Sep;85(9):1784-1790. doi: 10.1002/prot.25326. Epub 2017 Jun 7. PMID: 28544098.

9: Zhang F, Wei Q, Tong H, Xu D, Wang W, Ran T. Crystal structure of MBP-PigG
fusion protein and the essential function of PigG in the prodigiosin
biosynthetic pathway in Serratia marcescens FS14. Int J Biol Macromol. 2017
Jun;99:394-400. doi: 10.1016/j.ijbiomac.2017.02.088. Epub 2017 Mar 1. PMID:
28258005.

10: Xu D, Zhou J, Lou X, He J, Ran T, Wang W. Myroilysin Is a New Bacterial
Member of the M12A Family of Metzincin Metallopeptidases and Is Activated by a
Cysteine Switch Mechanism. J Biol Chem. 2017 Mar 31;292(13):5195-5206. doi:
10.1074/jbc.M116.758110. Epub 2017 Feb 9. PMID: 28188295; PMCID: PMC5392667.

11: Wu D, Li P, Zhou J, Gao M, Lou X, Ran T, Wu S, Wang W, Xu D. Identification
of a toxic serralysin family protease with unique thermostable property from S.
marcescens FS14. Int J Biol Macromol. 2016 Dec;93(Pt A):98-106. doi:
10.1016/j.ijbiomac.2016.08.041. Epub 2016 Aug 16. PMID: 27543346.

12: Wei Q, Ran T, Ma C, He J, Xu D, Wang W. Crystal Structure and Function of
PqqF Protein in the Pyrroloquinoline Quinone Biosynthetic Pathway. J Biol Chem.
2016 Jul 22;291(30):15575-87. doi: 10.1074/jbc.M115.711226. Epub 2016 May 26.
PMID: 27231346; PMCID: PMC4957043.

13: Zhou H, Li P, Wu D, Ran T, Wang W, Xu D. EheA from Exiguobacterium sp. yc3
is a novel thermostable DNase belonging to HNH endonuclease superfamily. FEMS
Microbiol Lett. 2015 Dec;362(24):fnv204. doi: 10.1093/femsle/fnv204. Epub 2015
Oct 22. PMID: 26500089.

14: Xu D, Gao Y, Wang P, Ran T, Wang W. Presence of an amino acid residue at
position 619 required for the function of YidC in Rhodobacter sphaeroides.
Biochem Biophys Res Commun. 2015 Oct 16;466(2):267-71. doi:
10.1016/j.bbrc.2015.09.032. Epub 2015 Sep 8. PMID: 26362178.

15: Li P, Kwok AH, Jiang J, Ran T, Xu D, Wang W, Leung FC. Comparative genome
analyses of Serratia marcescens FS14 reveals its high antagonistic potential.
PLoS One. 2015 Apr 9;10(4):e0123061. doi: 10.1371/journal.pone.0123061. PMID:
25856195; PMCID: PMC4391916.

16: Ran T, Gao M, Wei Q, He J, Tang L, Wang W, Xu D. Expression, crystallization
and preliminary crystallographic data analysis of VioD, a hydroxylase in the
violacein-biosynthesis pathway. Acta Crystallogr F Struct Biol Commun. 2015
Feb;71(Pt 2):149-52. doi: 10.1107/S2053230X14027617. Epub 2015 Jan 28. PMID:
25664787; PMCID: PMC4321467.

17: Han N, Ran T, Lou X, Gao Y, He J, Tang L, Xu D, Wang W. Expression,
crystallization and preliminary crystallographic data analysis of PigI, a
putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia.
Acta Crystallogr F Struct Biol Commun. 2014 May;70(Pt 5):624-7. doi:
10.1107/S2053230X14005780. Epub 2014 Apr 17. PMID: 24817724; PMCID: PMC4014333.

18: Lou X, Ran T, Han N, Gao Y, He J, Tang L, Xu D, Wang W. Crystal structure of
the catalytic domain of PigE: a transaminase involved in the biosynthesis of
2-methyl-3-n-amyl-pyrrole (MAP) from Serratia sp. FS14. Biochem Biophys Res
Commun. 2014 Apr 25;447(1):178-83. doi: 10.1016/j.bbrc.2014.03.125. Epub 2014
Apr 2. PMID: 24704447.

19: Ran T, Ozorowski G, Gao Y, Sineshchekov OA, Wang W, Spudich JL, Luecke H.
Cross-protomer interaction with the photoactive site in oligomeric
proteorhodopsin complexes. Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt
10):1965-80. doi: 10.1107/S0907444913017575. Epub 2013 Sep 20. PMID: 24100316.

20: Zhu W, Lan Y, Lou X, Han N, Ran T, Xu L, Xu D, Wang WW. Isolation of
proteorhodopsin-bearing bacterium JL-3 from fresh water and characterization of
the proteorhodopsin. FEMS Microbiol Lett. 2013 Jul;344(1):10-7. doi:
10.1111/1574-6968.12144. Epub 2013 May 1. PMID: 23551202.

21: Ran T, Gao Y, Marsh M, Zhu W, Wang M, Mao X, Xu L, Xu D, Wang W. Crystal
structures of Cg1458 reveal a catalytic lid domain and a common catalytic
mechanism for the FAH family. Biochem J. 2013 Jan 1;449(1):51-60. doi:
10.1042/BJ20120913. PMID: 23046410.

22: Liu S, Ran T, Shen X, Xu L, Wang W, Xu D. Expression, crystallization and
preliminary crystallographic data analysis of PigF, an O-methyltransferase from
the prodigiosin-synthetic pathway in Serratia. Acta Crystallogr Sect F Struct
Biol Cryst Commun. 2012 Aug 1;68(Pt 8):898-901. doi: 10.1107/S1744309112024001.
Epub 2012 Jul 31. PMID: 22869117; PMCID: PMC3412768.

23: Wang N, Wang M, Gao Y, Ran T, Lan Y, Wang J, Xu L, Wang W. Crystallization
and preliminary X-ray crystallographic analysis of a blue-light-absorbing
proteorhodopsin. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Mar
1;68(Pt 3):281-3. doi: 10.1107/S1744309111043612. Epub 2012 Feb 22. PMID:
22442222; PMCID: PMC3310530.

24: Ran T, Wang Y, Xu D, Wang W. Expression, purification, crystallization and
preliminary crystallographic analysis of Cg1458: a novel oxaloacetate
decarboxylase from Corynebacterium glutamicum. Acta Crystallogr Sect F Struct
Biol Cryst Commun. 2011 Aug 1;67(Pt 8):968-70. doi: 10.1107/S1744309111023220.
Epub 2011 Jul 27. PMID: 21821907; PMCID: PMC3151140.